Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Rabbit beta Actin Polyclonal Antibody | anti-ACTB antibody
beta Actin Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
beta Actin; Polyclonal Antibody; beta Actin Antibody; Affinity Purified Rabbit Polyclonal beta Actin Antibody; Polyclonal beta Actin antibody; Anti-beta Actin antibody; ACTB antibody; PS1TP5BP1 antibody; b-actin antibody; beta Actin antibody; beta-actin antibody; anti-ACTB antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
186
Applicable Applications for anti-ACTB antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:1000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Immunogen
beta Actin antibody was raised in rabbit using a synthetic peptide contain a sequence corresponding to a region within amino acids 309 and 358 of beta Actin
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Prostate using beta Actin antibody)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Prostate using beta Actin antibody)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10% SDS PAGE gel and beta Actin antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10% SDS PAGE gel and beta Actin antibody at a dilution of 1:1000)
Related Product Information for anti-ACTB antibody
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Product Categories/Family for anti-ACTB antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
42 kDa
NCBI Official Full Name
Beta-actin
Protein Family
Similar Products
Product Notes
The ACTB (Catalog #AAA5311771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's beta Actin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:1000 ICC: 1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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