Typical Testing Data/Standard Curve (for reference only)
Rat IRF4 (Interferon Regulatory Factor 4) ELISA Kit | IRF4 elisa kit
Rat IRF4 (Interferon Regulatory Factor 4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IRF4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IRF4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IRF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IRF4.You can calculate the concentration of IRF4in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
IRF4: Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Interacts with SPIB and DEF6. Not induced by interferons. Lymphoid cells. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Cellular Component: cytoplasm; membrane; nucleoplasm
Molecular Function: protein binding; protein-lysine N-methyltransferase activity; sequence-specific DNA binding; transcription factor activity
Biological Process: defense response to protozoan; myeloid dendritic cell differentiation; negative regulation of toll-like receptor signaling pathway; positive regulation of DNA binding; positive regulation of interleukin-10 biosynthetic process; positive regulation of interleukin-13 biosynthetic process; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid methylation
Research Articles on IRF4
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Product Notes
The Rat IRF4 irf4 (Catalog #AAA2540354) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2540354 ELISA Kit recognizes Rat IRF4. It is sometimes possible for the material contained within the vial of "IRF4 (Interferon Regulatory Factor 4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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