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Typical Testing Data/Standard Curve (for reference only)

Human RAGE ELISA Kit | RAGE elisa kit

RAGE Human ELISA Kit

Reactivity
Human
Applications
ELISA
Synonyms
RAGE; RAGE Human ELISA Kit; Human RAGE; RAGE elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Applicable Applications for RAGE elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of RAGE in human serum, plasma, urine, cell culture supernatant or tissue samples.
Samples
Rage in human serum, plasma, urine, cell culture supernatant or tissue samples
Assay Type
Quantitative Sandwich
Detection Range
130 pg/ml-4000 pg/ml
Preparation and Storage
Store at 2-8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RAGE elisa kit
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-RAGE antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-RAGE antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAGE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RAGE can be calculated.

Background/Introduction: RAGE, the Receptor for Advanced Glycation Endproducts, also called AGER, is a 35kD transmembrane receptor of the immunoglobulin super family which was first characterized in 1992 by Neeper et al. It is an important receptor for the amyloid beta peptide and that expression of this receptor increases in Alzheimer disease. Isoforms of the RAGE protein, which lack the transmembrane and the signaling domain (commonly referred to as soluble RAGE or sRAGE) are hypothesized to counteract the detrimental action of the full-length receptor and are hoped to provide a means to develop a cure against RAGE-associated diseases. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
RAGE protein

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Similar Products

Product Notes

The Human RAGE (Catalog #AAA355275) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355275 ELISA Kit recognizes Human RAGE. AAA Biotech's RAGE can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of RAGE in human serum, plasma, urine, cell culture supernatant or tissue samples. Researchers should empirically determine the suitability of the RAGE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAGE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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