Human Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit | PDIA3 elisa kit

Human Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit

Cat. Num. AAA2024248

• Gene Names: PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Reactivity: Human
• Synonyms: Protein Disulfide Isomerase A3 (PDIA3); Human Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit; P58; ERp61; ERp57; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein; 58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57; PDIA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A3 (PDIA3).; No significant cross-reactivity or interference between Protein Disulfide Isomerase A3 (PDIA3) and analogues was observed.
• Sequence Length: 505

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A3 (PDIA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A3 (PDIA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PDIA3 elisa kit

Signal transduction; Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 21361657
• NCBI GeneID: 2923
• NCBI Accession #: NP_005304.3
• NCBI GenBank Nucleotide #: NM_005313.4
• UniProt Accession #: P30101; Q13453; Q14255; Q8IYF8; Q9UMU7; AAB37397
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: protein disulfide-isomerase A3
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 3
• NCBI Official Symbol: PDIA3
• NCBI Official Synonym Symbols: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• NCBI Protein Information: protein disulfide-isomerase A3
• UniProt Protein Name: Protein disulfide-isomerase A3
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA3
• UniProt Synonym Gene Names: ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
• UniProt Entry Name: PDIA3_HUMAN

NCBI Description

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Protease; EC 5.3.4.1; Endoplasmic reticulum; Isomerase; Chaperone; Apoptosis; Phospholipase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: smooth endoplasmic reticulum; cell surface; focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; cell redox homeostasis; protein import into nucleus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; proteolysis; signal transduction; protein retention in ER