Rabbit anti-Human, Mouse Atrial Natriuretic Peptide Polyclonal Antibody | anti-ANP antibody

Atrial Natriuretic Peptide, NT (ANP, Atrial Natriuretic Factor, ANF, ATFB6, Cardiodilatin-related Peptide, CDP, CDD-ANF, Natriuretic Peptide Precursor A, NPPA, Prepronatriodilatin, PND) (PE)

Cat. Num. AAA6464473

• Gene Names: NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Atrial Natriuretic Peptide; Polyclonal Antibody; NT (ANP; Atrial Natriuretic Factor; ANF; ATFB6; Cardiodilatin-related Peptide; CDP; CDD-ANF; Natriuretic Peptide Precursor A; NPPA; Prepronatriodilatin; PND) (PE); anti-ANP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NPPA. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ANP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human NPPA (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NPPA Antibody (N-term) in mouse heart tissue lysates (35ug/lane). NPPA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma with NPPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using NPPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ANP antibody

Antibodies; Cardiac Markers

References

1. Watanabe, Y., et.al., Biochem. Mol. Med. 61(1), 47-51 (1997). 2. Suga, S., et.al., Endocrinology 130(1), 229-239 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 23510319
• NCBI GeneID: 4878
• NCBI Accession #: NP_006163.1
• NCBI GenBank Nucleotide #: NM_006172.3
• UniProt Accession #: P01160; Q13766; Q5JZE1
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: natriuretic peptides A preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A
• UniProt Protein Name: Natriuretic peptides A
• Protein Family: Andropin
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDP; ANF; ANP
• UniProt Entry Name: ANF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor binding

Biological Process: cardiac muscle hypertrophy; transcription initiation from RNA polymerase II promoter; cGMP biosynthetic process; negative regulation of systemic arterial blood pressure; positive regulation of heart rate; female pregnancy; response to insulin stimulus; neuropeptide signaling pathway; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; response to hypoxia; gene expression; negative regulation of cell growth; regulation of blood vessel size

Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6