Rabbit anti-Human AGPAT5 Polyclonal Antibody | anti-AGPAT5 antibody

AGPAT5, NT (AGPAT5, 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon) (FITC)

Cat. Num. AAA6272259

• Gene Names: AGPAT5; LPAATE; 1AGPAT5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AGPAT5; Polyclonal Antibody; NT (AGPAT5; 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-acylglycerol-3-phosphate O-acyltransferase 5; Lysophosphatidic acid acyltransferase epsilon) (FITC); Lysophosphatidic acid acyltransferase epsilon; anti-AGPAT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AGPAT5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AGPAT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 27-54 amino acids selected from the N-terminal region of human AGPAT5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AGPAT5 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AGPAT5 antibody detected the AGPAT5 protein (arrow).)

Related Product Information for anti-AGPAT5 antibody

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.

Product Categories/Family for anti-AGPAT5 antibody

Antibodies; Enzymes; Acyltransferase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Agarwal, A.K., et al. Arch. Biochem. Biophys. 449 (1-2), 64-76 (2006): Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Lu, B., et al. Biochem. J. 385 (PT 2), 469-477 (2005): Leung, D.W. Front. Biosci. 6, D944-D953 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 61743952
• NCBI GeneID: 55326
• NCBI Accession #: NP_060831.2
• NCBI GenBank Nucleotide #: NM_018361.3
• UniProt Accession #: Q9NUQ2; Q8IZ47; Q9BQG4
• Molecular Weight: 42,072 Da
• NCBI Official Full Name: 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon
• NCBI Official Synonym Full Names: 1-acylglycerol-3-phosphate O-acyltransferase 5
• NCBI Official Symbol: AGPAT5
• NCBI Official Synonym Symbols: LPAATE; 1AGPAT5
• NCBI Protein Information: 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-AGPAT 5; 1-AGP acyltransferase 5; lysophosphatidic acid acyltransferase epsilon; 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon)
• UniProt Protein Name: 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon
• Protein Family: 1-acyl-sn-glycerol-3-phosphate acyltransferase
• UniProt Gene Name: AGPAT5
• UniProt Synonym Gene Names: 1-AGP acyltransferase 5; 1-AGPAT 5
• UniProt Entry Name: PLCE_HUMAN

NCBI Description

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

AGPAT5: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Membrane protein, integral; EC 2.3.1.51; Transferase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; integral to membrane; nuclear envelope

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; hemopoietic progenitor cell differentiation; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process