Mouse anti-Human MAGEB1 Monoclonal Antibody | anti-MAGEB1 antibody

MAGEB1 (Melanoma-associated Antigen B1, MAGE-B1 Antigen, MAGE-XP Antigen, DSS-AHC Critical Interval MAGE Superfamily 10, DAM10, Cancer/Testis Antigen 3.1, CT3.1, MAGEL1, MAGEXP) (FITC)

Cat. Num. AAA6148092

• Gene Names: MAGEB1; CT3.1; DAM10; MAGEL1; MAGE-Xp
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAGEB1; Monoclonal Antibody; MAGEB1 (Melanoma-associated Antigen B1; MAGE-B1 Antigen; MAGE-XP Antigen; DSS-AHC Critical Interval MAGE Superfamily 10; DAM10; Cancer/Testis Antigen 3.1; CT3.1; MAGEL1; MAGEXP) (FITC); anti-MAGEB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2A4
• Specificity: Recognizes human MAGEB1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-MAGEB1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-347 from human MAGEB1 (AAH13772) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MPRGQKSKLRAREKRRKAREETQGLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRAKRRTTATTFRARSRAPFSRSSHPM
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (63.91kD).)

Western Blot (WB)

(Western Blot analysis of MAGEB1 expression in transfected 293T cell line by MAGEB1 monoclonal antibody. Lane 1: MAGEB1 transfected lysate (Predicted MW: 39kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged MAGEB1 is ~1ng/ml as a capture antibody.)

Related Product Information for anti-MAGEB1 antibody

MAGEB1 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21.

Product Categories/Family for anti-MAGEB1 antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 15489350
• NCBI GeneID: 4112
• Molecular Weight: 39,037 Da
• NCBI Official Full Name: Homo sapiens melanoma antigen family B, 1, mRNA
• NCBI Official Synonym Full Names: MAGE family member B1
• NCBI Official Symbol: MAGEB1
• NCBI Official Synonym Symbols: CT3.1; DAM10; MAGEL1; MAGE-Xp
• NCBI Protein Information: melanoma-associated antigen B1
• Protein Family: Melanoma-associated antigen

NCBI Description

This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]