Human Glycodelin ELISA Kit | PAEP elisa kit

Human Glycodelin ELISA Kit

Cat. Num. AAA2884971

• Gene Names: PAEP; GD; GdA; GdF; GdS; PEP; PAEG; PP14
• Reactivity: Human
• Synonyms: Glycodelin; Human Glycodelin ELISA Kit; GD; Placental protein 14; PP14; Pregnancy-associated endometrial alpha-2 globulin; PAEG; PEG; Progestagen-associated endometrial protein; Progesterone-associated endometrial; PAEP elisa kit

• Reactivity: Human
• Sequence Length: 158

• Assay Type: Sandwich
• Detection Range: 3.12-200 ng/mL
• Sensitivity: 0.78 ng/ml
• Intra-Assay CV: <=4.3%
• Inter-Assay CV: <=7.9%
• Recovery: 102%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAEP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 937226996
• NCBI GeneID: 5047
• NCBI Accession #: NP_001018058.1
• NCBI GenBank Nucleotide #: NM_001018048.1
• UniProt Accession #: P09466; Q5T6T1; Q9UG92
• Molecular Weight: 9,953 Da
• NCBI Official Full Name: glycodelin isoform 2
• NCBI Official Synonym Full Names: progestagen-associated endometrial protein
• NCBI Official Symbol: PAEP
• NCBI Official Synonym Symbols: GD; GdA; GdF; GdS; PEP; PAEG; PP14
• NCBI Protein Information: glycodelin
• UniProt Protein Name: Glycodelin
• Protein Family: Glycodelin
• UniProt Gene Name: PAEP
• UniProt Synonym Gene Names: GD; PP14; PAEG; PEG
• UniProt Entry Name: PAEP_HUMAN

NCBI Description

This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PAEP: This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Belongs to the calycin superfamily. Lipocalin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular region

Molecular Function: protein binding

Biological Process: apoptosis; multicellular organismal development; positive regulation of granulocyte macrophage colony-stimulating factor production; transport