Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit | POLd elisa kit

Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit

Cat. Num. AAA2704632

• Gene Names: POLD1; CDC2; MDPL; POLD; CRCS10
• Reactivity: Human
• Synonyms: Polymerase DNA Directed Delta 1 (POLd); Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit; CDC2; POLD1; DNA polymerase subunit delta p125; POLd elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Delta 1 (POLd).; No significant cross-reactivity or interference between Polymerase DNA Directed Delta 1 (POLd) and analogues was observed.
• Sequence Length: 1,107

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POLd elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Delta 1 (POLd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Delta 1 (POLd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Delta 1 (POLd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Delta 1 (POLd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14250670
• NCBI GeneID: 5424
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: Polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• NCBI Official Synonym Full Names: DNA polymerase delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; MDPL; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).