Western Blot (WB)
(The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysate)
Rabbit anti-Human Phospho-CDC25B (S353) Polyclonal Antibody | anti-CDC25B antibody
Phospho-CDC25B (S353) Antibody
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-CDC25B (S353); Polyclonal Antibody; Phospho-CDC25B (S353) Antibody; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2; anti-CDC25B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDC25B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S353 of human CDC25B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Length
566
Applicable Applications for anti-CDC25B antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysate)
Western Blot (WB)
(The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysate)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma)
Related Product Information for anti-CDC25B antibody
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
Product Categories/Family for anti-CDC25B antibody
References
Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004). Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003). Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003). Theis-Febvre, N., et al., Oncogene 22(2):220-232 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64987
NCBI Official Full Name
M-phase inducer phosphatase 2 isoform 2
NCBI Official Synonym Full Names
cell division cycle 25B
NCBI Official Symbol
CDC25B
NCBI Protein Information
M-phase inducer phosphatase 2
UniProt Protein Name
M-phase inducer phosphatase 2
UniProt Gene Name
CDC25B
UniProt Synonym Gene Names
CDC25HU2
UniProt Entry Name
MPIP2_HUMAN
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Product Notes
The CDC25B cdc25b (Catalog #AAA9206991) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CDC25B (S353) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-CDC25B (S353) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CDC25B cdc25b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-CDC25B (S353), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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