POLD1 recombinant protein

Recombinant Human POLD1 protein

Cat. Num. AAA1562734

• Gene Names: POLD1; CDC2; POLD; CRCS10
• Purity: >90% as determined by SDS-PAGE
• Synonyms: POLD1; Recombinant Human POLD1 protein; DNA polymerase delta catalytic subunit; 3'-5' exodeoxyribonuclease; DNA polymerase subunit delta p125; POLD1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human POLD1 (Met41-Phe254) was fused with GST tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for POLD1 recombinant protein

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites.

Product Categories/Family for POLD1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 379030588
• NCBI GeneID: 5424
• NCBI Accession #: NP_001243778.1
• NCBI GenBank Nucleotide #: NM_001256849.1
• UniProt Accession #: P28340; Q8NER3; Q96H98
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: DNA polymerase delta catalytic subunit
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: EC 2.7.7.7; Transferase; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Hydrolase; Nucleotide Metabolism - purine; DNA replication

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; delta DNA polymerase complex; nucleotide-excision repair complex; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; 3'-5'-exodeoxyribonuclease activity; chromatin binding

Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; DNA replication proofreading; nucleotide-excision repair; base-excision repair; fatty acid homeostasis; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA catabolic process, exonucleolytic; telomere maintenance; response to UV

Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome