Rabbit anti-Human ADAT3 Polyclonal Antibody | anti-ADAT3 antibody

Anti-ADAT3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102068

• Gene Names: ADAT3; TAD3; MRT36; FWP005; MST121; S863-5; MSTP121
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: ADAT3; Polyclonal Antibody; Anti-ADAT3 Antibody; Rabbit Polyclonal; ADAT3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-FWP005 Antibody; Anti-MRT36 Antibody; Anti-MST121 Antibody; Anti-MSTP121 Antibody; Anti-S863-5 Antibody; Anti-TAD3 Antibody; anti-ADAT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ADAT3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-ADAT3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human ADAT3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ADAT3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ADAT3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:38 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human ADAT3 in human adrenal gland with rabbit polyclonal antibody at 1:1500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-ADAT3 antibody

Adenosine (A)-to-inosine (I) RNA editing is a post-transcriptional RNA process capable of generating RNA and protein diversity. Inosine at wobble position 34 of tRNA anticodons can translate codons ending in uracil, cytosine, or adenine. The modification, which creates an I from an A at position 34 (wobble position) of tRNA, is catalyzed by the heterodimeric enzyme, adenosine deaminase, tRNA-specific 3 (ADAT3)/ADAT2. In humans, a valine-to-methionine mutation (V144M) in ADAT3 that originated ~1, 600 years ago is the most common cause of autosomal recessive intellectual disability (ID) in Arabia.

References

Salehi Chaleshtori AR, et al. (2018) A novel 8-bp duplication in adat3 causes mild intellectual disability. Hum Genome Var 5 7.Ramos J, et al. (2019) Formation of trna wobble inosine in humans is disrupted by a millennia-old mutation causing intellectual disability. Mol Cell Biol 39 (19): e00203-19.

NCBI and Uniprot Product Information

• NCBI GI #: 400153497
• NCBI GeneID: 113179
• NCBI Accession #: NP_612431.2
• NCBI GenBank Nucleotide #: NM_138422.2
• UniProt Accession #: Q96EY9
• Molecular Weight: 38,071 Da
• NCBI Official Full Name: probable inactive tRNA-specific adenosine deaminase-like protein 3
• NCBI Official Synonym Full Names: adenosine deaminase, tRNA-specific 3
• NCBI Official Symbol: ADAT3
• NCBI Official Synonym Symbols: TAD3; MRT36; FWP005; MST121; S863-5; MSTP121
• NCBI Protein Information: probable inactive tRNA-specific adenosine deaminase-like protein 3; tRNA-specific adenosine deaminase 3 homolog; adenosine deaminase, tRNA-specific 3, TAD3 homolog; tRNA-specific adenosine-34 deaminase subunit ADAT3
• UniProt Protein Name: Probable inactive tRNA-specific adenosine deaminase-like protein 3
• Protein Family: Probable inactive tRNA-specific adenosine deaminase-like protein
• UniProt Gene Name: ADAT3
• UniProt Synonym Gene Names: TAD3
• UniProt Entry Name: ADAT3_HUMAN