Human Phospholipase D2 (PLD2) ELISA Kit | PLD2 elisa kit

Human Phospholipase D2 (PLD2) ELISA Kit

Cat. Num. AAA2705267

• Gene Names: PLD2; PLD1C
• Reactivity: Human
• Synonyms: Phospholipase D2 (PLD2); Human Phospholipase D2 (PLD2) ELISA Kit; PLD1C; Choline Phosphatase 2; Phosphatidylcholine-hydrolyzing phospholipase D2; PLD2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase D2 (PLD2).; No significant cross-reactivity or interference between Phospholipase D2 (PLD2) and analogues was observed.
• Sequence Length: 922

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.55ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase D2 (PLD2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase D2 (PLD2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLD2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D2 (PLD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase D2 (PLD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase D2 (PLD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase D2 (PLD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2773044
• NCBI GeneID: 5338
• UniProt Accession #: O14939; O43540; O43579; O43580; Q6PGR0; Q96BY3; I3L2C9
• Molecular Weight: 104,657 Da
• NCBI Official Full Name: phospholipase D2
• NCBI Official Synonym Full Names: phospholipase D2
• NCBI Official Symbol: PLD2
• NCBI Official Synonym Symbols: PLD1C
• NCBI Protein Information: phospholipase D2
• UniProt Protein Name: Phospholipase D2
• Protein Family: Phospholipase
• UniProt Gene Name: PLD2
• UniProt Synonym Gene Names: PLD 2; hPLD2

NCBI Description

The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.