Human
Tissue, Total RNA Adult Normal, Skin, BioGenomics RNA Extraction
Tissue, Total RNA, Human Adult Normal, Skin, BioGenomics
Purity
Total RNA isolated by modified guanidine thiocyanate techniques.
Synonyms
Tissue; Total RNA Adult Normal; Skin; BioGenomics; Total RNA; Human Adult Normal; BioGenomics rna extraction
Purity/Purification
Total RNA isolated by modified guanidine thiocyanate techniques.
Form/Format
Supplied as a liquid in RNA Storage Buffer: 0.1% DEPC-treated water with 0.1mM EDTA. No non-specific endonuleases, no nickase, no exonuclease and no RNase detected.
Application Notes
Suitable for use in Northern Blot, RT-PCR, RACE, cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection and primer extension, RNA display, Purification of mRNA.
Features
Total RNA isolated from a wide variety of hard to obtain tissues
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Available documentation of tissues' clinical histories (additional cost)
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Available documentation of tissues' clinical histories (additional cost)
Quality Control
1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18S and 28S ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/A280 is 1.8-2.0 (detected in 10mM Tris-Cl, pH 7.5).
2. The RNA is treated by DNase I. It is tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
2. The RNA is treated by DNase I. It is tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
Donor Information
RNA from one normal, 76 year old, male, adult human (DNase 1 treated)
Preparation and Storage
Ships oon dry ice. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Human
Human
Related Product Information for Tissue, Total RNA Adult Normal, Skin, BioGenomics rna extraction
Total RNAs are available from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, and RNA differential display.
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Product Notes
The Tissue, Total RNA Adult Normal, Skin, BioGenomics (Catalog #AAA638534) is a RNA Extraction and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in Northern Blot, RT-PCR, RACE, cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection and primer extension, RNA display, Purification of mRNA. Researchers should empirically determine the suitability of the Tissue, Total RNA Adult Normal, Skin, BioGenomics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tissue, Total RNA Adult Normal, Skin, BioGenomics, RNA Extraction" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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