Typical Testing Data/Standard Curve (for reference only)
Human Phospholipase A2, Group X (PLA2G10) ELISA Kit | PLA2G10 elisa kit
Human Phospholipase A2, Group X (PLA2G10) ELISA Kit
Gene Names
PLA2G10; SPLA2; GXPLA2; GXSPLA2; sPLA2-X
Reactivity
Human
Synonyms
Phospholipase A2; Group X (PLA2G10); Human Phospholipase A2; Group X (PLA2G10) ELISA Kit; GXPLA2; GX sPLA2; sPLA2-X; Group 10 secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 10; PLA2G10 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PLA2G10. No significant cross-reactivity or interference between PLA2G10 and analogues was observed.
Sequence Length
165
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
37pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLA2G10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLA2G10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G10 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLA2G10 in human serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLA2G10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLA2G10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,153 Da
NCBI Official Full Name
group 10 secretory phospholipase A2 preproprotein
NCBI Official Synonym Full Names
phospholipase A2 group X
NCBI Official Symbol
PLA2G10
NCBI Official Synonym Symbols
SPLA2; GXPLA2; GXSPLA2; sPLA2-X
NCBI Protein Information
group 10 secretory phospholipase A2
UniProt Protein Name
Group 10 secretory phospholipase A2
Protein Family
UniProt Gene Name
PLA2G10
UniProt Synonym Gene Names
GX sPLA2; sPLA2-X
NCBI Description
This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism. [provided by RefSeq, Nov 2015]
Uniprot Description
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
Research Articles on PLA2G10
1. Data show that phospholipase A2 group IIA, V and X have different target/function related activity.
Similar Products
Product Notes
The Human PLA2G10 pla2g10 (Catalog #AAA452013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA452013 ELISA Kit recognizes Human PLA2G10. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Group X (PLA2G10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
