Human Phospholipase A2, Group X ELISA Kit | PLA2G10 elisa kit

Human Phospholipase A2, Group X ELISA Kit

Cat. Num. AAA760636

• Gene Names: PLA2G10; SPLA2; GXPLA2; GXSPLA2; sPLA2-X
• Reactivity: Human
• Synonyms: Phospholipase A2; Group X; Human Phospholipase A2; Group X ELISA Kit; PLA2G10; PLA2G10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PL. No significant cross-reactivity or interference between PL and analogues was observed.
• Sequence Length: 165

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000ng/ml
• Sensitivity: <46.875ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G10 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 109730371
• NCBI GeneID: 8399
• Molecular Weight: 18,153 Da
• NCBI Official Full Name: Phospholipase A2, group X
• NCBI Official Synonym Full Names: phospholipase A2 group X
• NCBI Official Symbol: PLA2G10
• NCBI Official Synonym Symbols: SPLA2; GXPLA2; GXSPLA2; sPLA2-X
• NCBI Protein Information: group 10 secretory phospholipase A2
• UniProt Protein Name: Group 10 secretory phospholipase A2
• Protein Family: Group 10 secretory phospholipase
• UniProt Gene Name: PLA2G10
• UniProt Synonym Gene Names: GX sPLA2; sPLA2-X
• UniProt Entry Name: PA2GX_HUMAN

NCBI Description

This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism. [provided by RefSeq, Nov 2015]

Uniprot Description

PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family.

Protein type: Secreted; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Cell development/differentiation; Phospholipase; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.1-p12

Cellular Component: extracellular region

Molecular Function: calcium ion binding; phospholipase A2 activity; phospholipase activity

Biological Process: arachidonic acid metabolic process; axon guidance; cholesterol homeostasis; glycerophospholipid biosynthetic process; lipid catabolic process; lysophospholipid transport; negative regulation of transcription factor activity; phosphatidic acid biosynthetic process; phospholipid metabolic process; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; regulation of macrophage activation