Typical Testing Data/Standard Curve (for reference only)
Chicken PLA2G10 ELISA Kit | PLA2G10 elisa kit
Chicken PLA2G10 (Phospholipase A2, Group X) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLA2G10. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLA2G10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLA2G10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLA2G10. You can calculate the concentration of PLA2G10 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family.
Protein type: Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis; Phospholipase; Secreted; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Cell development/differentiation
Chromosomal Location of Human Ortholog: 16p13.1-p12
Cellular Component: extracellular region
Molecular Function: phospholipase A2 activity; calcium ion binding; phospholipase activity
Biological Process: axon guidance; cholesterol homeostasis; phospholipid metabolic process; lysophospholipid transport; regulation of macrophage activation; negative regulation of transcription factor activity; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; lipid catabolic process
Research Articles on PLA2G10
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Product Notes
The Chicken PLA2G10 pla2g10 (Catalog #AAA2505031) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505031 ELISA Kit recognizes Chicken PLA2G10. It is sometimes possible for the material contained within the vial of "PLA2G10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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