Human Phospholipase A1 (PLA1) ELISA Kit | PLA1 elisa kit

Human Phospholipase A1 (PLA1) ELISA Kit

Cat. Num. AAA454186

• Gene Names: PLA1A; PSPLA1; PS-PLA1
• Reactivity: Human
• Synonyms: Phospholipase A1 (PLA1); Human Phospholipase A1 (PLA1) ELISA Kit; PLA1A; ps-PLA1; NMD; Phosphatidylserine-Specific Phospholipase A1-Alpha; PLA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PKM2. No significant cross-reactivity or interference between PKM2 and analogues was observed.
• Sequence Length: 440

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 29pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PKM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PKM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PKM2 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PKM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PKM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 332688256
• NCBI GeneID: 51365
• NCBI Accession #: NP_001193889.1
• NCBI GenBank Nucleotide #: NM_001206960.1
• UniProt Accession #: Q53H76; O95991; Q86WX6; Q9UPD2; B2R8V2; B4DXA2
• Molecular Weight: 31,285 Da
• NCBI Official Full Name: phospholipase A1 member A isoform 2
• NCBI Official Synonym Full Names: phospholipase A1 member A
• NCBI Official Symbol: PLA1A
• NCBI Official Synonym Symbols: PSPLA1; PS-PLA1
• NCBI Protein Information: phospholipase A1 member A
• UniProt Protein Name: Phospholipase A1 member A
• Protein Family: Phospholipase
• UniProt Gene Name: PLA1A
• UniProt Synonym Gene Names: NMD; PSPLA1; PS-PLA1

NCBI Description

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.