Western Blot (WB)
(Anti-TFPI2 antibody, MBS175398, Western blottingLane 1: MM453 Cell LysateLane 2: MM231 Cell LysateLane 3: HELA Cell LysateLane 4: HT1080 Cell LysateLane 5: JURKAT Cell Lysate )
Rabbit anti-Human TFPI2 Polyclonal Antibody | anti-TFPI2 antibody
Anti-TFPI2 antibody
Gene Names
TFPI2; PP5; REF1; TFPI-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
TFPI2; Polyclonal Antibody; Anti-TFPI2 antibody; Tissue factor pathway inhibitor 2(TFPI-2); tissue factor pathway inhibitor 2; Placental protein 5 antibody; PP5 antibody; TFPI 2 antibody; TFPI-2 antibody; TFPI2 antibody; TFPI2 PEN antibody; TFPI2_HUMAN antibody; Tissue factor pathway inhibitor 2 antibody; anti-TFPI2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
235
Applicable Applications for anti-TFPI2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Immuohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Immuohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TFPI2 (170-186aa NVTRYYFNPRYRTCDAF).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-TFPI2 antibody, MBS175398, Western blottingLane 1: MM453 Cell LysateLane 2: MM231 Cell LysateLane 3: HELA Cell LysateLane 4: HT1080 Cell LysateLane 5: JURKAT Cell Lysate )
Western Blot (WB)
(Anti-TFPI2 antibody, MBS175398, Western blottingLane 1: MM453 Cell LysateLane 2: MM231 Cell LysateLane 3: HELA Cell LysateLane 4: HT1080 Cell LysateLane 5: JURKAT Cell Lysate )
Immunohistochemistry (IHC)
(Anti-TFPI2 antibody, MBS175398, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-TFPI2 antibody, MBS175398, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Related Product Information for anti-TFPI2 antibody
Description: Rabbit IgG polyclonal antibody for Tissue factor pathway inhibitor 2(TFPI2) detection. Tested with WB, IHC-P in Human.
Background: Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.
Background: Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,796 Da
NCBI Official Full Name
Tissue factor pathway inhibitor 2
NCBI Official Synonym Full Names
tissue factor pathway inhibitor 2
NCBI Official Symbol
TFPI2
NCBI Official Synonym Symbols
PP5; REF1; TFPI-2
NCBI Protein Information
tissue factor pathway inhibitor 2; placental protein 5; retinal pigment epithelium cell factor 1
UniProt Protein Name
Tissue factor pathway inhibitor 2
Protein Family
UniProt Gene Name
TFPI2
UniProt Synonym Gene Names
TFPI-2; PP5
UniProt Entry Name
TFPI2_HUMAN
NCBI Description
This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: proteinaceous extracellular matrix; nucleus
Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent
Biological Process: blood coagulation
Research Articles on TFPI2
Similar Products
Product Notes
The TFPI2 tfpi2 (Catalog #AAA175398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TFPI2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TFPI2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu Immuohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Hu Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the TFPI2 tfpi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFPI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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