Human OTUD5 (OTU domain containing 5) ELISA Kit | OTUD5 elisa kit

Human OTUD5 (OTU domain containing 5) ELISA Kit

Cat. Num. AAA7608221

• Gene Names: OTUD5; DUBA
• Reactivity: Human
• Synonyms: OTUD5 (OTU domain containing 5); Human OTUD5 (OTU domain containing 5) ELISA Kit; Deubiquitinating enzyme A; DUBA; OTU domain containing 5; OTUD5; OTUD5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of OTUD5. No significant cross-reactivity or interference between OTUD5 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OTUD5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119571108
• NCBI GeneID: 55593
• UniProt Accession #: Q96G74; Q4KMN9; Q8N6T5; Q9H650; Q9H9U0; Q9NT65; B4DGG7; G5E9D7
• Molecular Weight: 60,210 Da
• NCBI Official Full Name: OTU domain containing 5, isoform CRA_b
• NCBI Official Synonym Full Names: OTU deubiquitinase 5
• NCBI Official Symbol: OTUD5
• NCBI Official Synonym Symbols: DUBA
• NCBI Protein Information: OTU domain-containing protein 5; OTU domain containing 5; deubiquinating enzyme A; deubiquitinating enzyme A
• UniProt Protein Name: OTU domain-containing protein 5
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD5
• UniProt Synonym Gene Names: DUBA
• UniProt Entry Name: OTUD5_HUMAN

NCBI Description

This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

OTUD5: a protein of unknown function. Contains a OTU-like cysteine protease domain.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytosol

Molecular Function: ubiquitin-specific protease activity

Biological Process: innate immune response; response to lipopolysaccharide; proteolysis; negative regulation of interferon type I production