Human 5-oxoprolinase ELISA Kit | OPLAH elisa kit

Human 5-oxoprolinase ELISA Kit

Cat. Num. AAA761348

• Gene Names: OPLAH; OPLA; OPLAHD; 5-Opase
• Reactivity: Human
• Synonyms: 5-oxoprolinase; Human 5-oxoprolinase ELISA Kit; OPLAH/5-oxo-L-prolinase (5-OPase)/Pyroglutamase; OPLAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of OPLAH. No significant cross-reactivity or interference between OPLAH and analogues was observed.
• Sequence Length: 1288

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for OPLAH elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 48314820
• NCBI GeneID: 26873
• NCBI Accession #: NP_060040.1
• NCBI GenBank Nucleotide #: NM_017570.4
• Molecular Weight: 137,457 Da
• NCBI Official Full Name: 5-oxoprolinase
• NCBI Official Synonym Full Names: 5-oxoprolinase (ATP-hydrolysing)
• NCBI Official Symbol: OPLAH
• NCBI Official Synonym Symbols: OPLA; OPLAHD; 5-Opase
• NCBI Protein Information: 5-oxoprolinase
• UniProt Protein Name: 5-oxoprolinase
• Protein Family: 5-oxoprolinase
• UniProt Gene Name: OPLAH
• UniProt Synonym Gene Names: 5-OPase
• UniProt Entry Name: OPLA_HUMAN

NCBI Description

The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). [provided by RefSeq, Jun 2012]

Uniprot Description

OPLAH: Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Defects in OPLAH are the cause of 5-oxoprolinase deficiency (OPLAHD). A disorder characterized by calcium oxalate/carbonate urolithiasis, and excessive urinary 5- oxo-L-proline. Affected individuals have recurrent episodes of vomiting, diarrhea, and abdominal pain. Belongs to the oxoprolinase family.

Protein type: Hydrolase; EC 3.5.2.9; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: 5-oxoprolinase (ATP-hydrolyzing) activity; ATP binding

Biological Process: glutathione biosynthetic process; xenobiotic metabolic process

Disease: 5-oxoprolinase Deficiency