Human MBD3 (Methyl-CpG-binding domain protein 3) ELISA Kit | MBD3 elisa kit

Human MBD3 (Methyl-CpG-binding domain protein 3) ELISA Kit

Cat. Num. AAA7608233

• Reactivity: Human
• Synonyms: MBD3 (Methyl-CpG-binding domain protein 3); Human MBD3 (Methyl-CpG-binding domain protein 3) ELISA Kit; MBD3; Methyl-CpG-binding domain protein 3; MBD3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MBD3. No significant cross-reactivity or interference between MBD3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBD3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505119
• NCBI GeneID: 53615
• NCBI Accession #: NP_003917.1
• NCBI GenBank Nucleotide #: NM_003926.5
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
• Molecular Weight: 32,844 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3
• UniProt Entry Name: MBD3_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq, Jul 2008]

Uniprot Description

MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

Protein type: Gene silencing; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; heterochromatin; protein complex; nuclear chromatin; cytoplasm; NuRD complex

Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding

Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; methylation-dependent chromatin silencing; in utero embryonic development; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; histone acetylation