Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) Standard Curve.)
Human Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor epsilon (NFKBIE) ELISA Kit | NFKBIE elisa kit
Human Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor epsilon (NFKBIE) ELISA Kit
Gene Names
NFKBIE; IKBE
Reactivity
Human
Synonyms
Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor epsilon (NFKBIE); Human Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor epsilon (NFKBIE) ELISA Kit; NFKBIE; IKBE; NFKBIE elisa kit
Reactivity
Human
Specificity
Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) ELISA Kit has high sensitivity and excellent specificity for detection of Human NFKBIE. No significant cross-reactivity or interference between Human NFKBIE and analogues was observed.
Sequence Length
500
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) Standard Curve.)
Related Product Information for NFKBIE elisa kit
Description: This Human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKBIE in samples. An antibody specific for NFKBIE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NFKBIE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKBIE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKBIE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NFKBIE protein expression is up-regulated following NF-kappaB activation and during myelopoiesis. NFKBIE is able to inhibit NF-kappaB-directed transactivation via cytoplasmic retention of REL proteins.NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-kappaB transcription factor complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex.For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).
Background: NFKBIE protein expression is up-regulated following NF-kappaB activation and during myelopoiesis. NFKBIE is able to inhibit NF-kappaB-directed transactivation via cytoplasmic retention of REL proteins.NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-kappaB transcription factor complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex.For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NF-kappa-B inhibitor epsilon
NCBI Official Synonym Full Names
NFKB inhibitor epsilon
NCBI Official Symbol
NFKBIE
NCBI Official Synonym Symbols
IKBE
NCBI Protein Information
NF-kappa-B inhibitor epsilon
UniProt Protein Name
NF-kappa-B inhibitor epsilon
Protein Family
UniProt Gene Name
NFKBIE
UniProt Synonym Gene Names
IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon
UniProt Entry Name
IKBE_HUMAN
NCBI Description
The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]
Uniprot Description
IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.
Protein type: Inhibitor; DNA-binding
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; cytosol
Molecular Function: protein binding
Biological Process: cytoplasmic sequestering of transcription factor; D-serine transport
Research Articles on NFKBIE
Similar Products
Product Notes
The Human NFKBIE nfkbie (Catalog #AAA9716199) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716199 ELISA Kit recognizes Human NFKBIE. It is sometimes possible for the material contained within the vial of "Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor epsilon (NFKBIE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
