Typical Standard Curve/Testing Data
(Human NT-proANP/NPPA PicoKine ELISA Kit standard curve)
Human NT-proANP/NPPA ELISA Kit | NPPA elisa kit
Human NT-proANP/NPPA PicoKine ELISA Kit
Gene Names
NPPA; ANF; ANP
Reactivity
Human
Synonyms
NT-proANP/NPPA; Human NT-proANP/NPPA PicoKine ELISA Kit; Human NPPA; NPPA elisa kit
Reactivity
Human
Specificity
Natural and recombinant human NPPA
Sequence Length
149
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Capture Antibody
A monoclonal antibody from mouse.
Detection Antibody
A biotinylated polyclonal antibody from goat.
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |90|200|925|91|182|898
Standard Deviation: |4.23|13.8|52.72|5.46|15.83|66.45
CV(%): |4.7%|6.9%|5.7%|6%|8.7%|7.4%
n|16|16|16|24|24|24
Mean(pg/ml): |90|200|925|91|182|898
Standard Deviation: |4.23|13.8|52.72|5.46|15.83|66.45
CV(%): |4.7%|6.9%|5.7%|6%|8.7%|7.4%
Sample Data
Concentration (pg/ml): |0|31.2|62.5|125|250|500|1000|2000
OD: |0.094|0.158|0.228|0.376|0.669|1.101|1.812|2.414
OD: |0.094|0.158|0.228|0.376|0.669|1.101|1.812|2.414
Typical Standard Curve/Testing Data
(Human NT-proANP/NPPA PicoKine ELISA Kit standard curve)
Typical Standard Curve/Testing Data
(Human NT-proANP/NPPA PicoKine ELISA Kit standard curve)
Related Product Information for NPPA elisa kit
Description: For quantitative detection of human NPPA in cell culture supernates, serum and plasma (heparin, EDTA).
Background: Human NPPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NPPA has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: N26-R123) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NPPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NPPA amount of sample captured in plate.
Background: Human NPPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NPPA has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: N26-R123) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NPPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NPPA amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
natriuretic peptides A
NCBI Official Synonym Full Names
natriuretic peptide A
NCBI Official Symbol
NPPA
NCBI Official Synonym Symbols
ANF; ANP
NCBI Protein Information
natriuretic peptides A
Research Articles on NPPA
Similar Products
Product Notes
The Human NPPA (Catalog #AAA1751565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751565 ELISA Kit recognizes Human NPPA. It is sometimes possible for the material contained within the vial of "NT-proANP/NPPA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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