Typical Standard Curve/Testing Data
(Mouse AAT PicoKine ELISA Kit standard curve)
Mouse Serpin A1/AAT ELISA Kit | AAT elisa kit
Mouse Serpin A1/AAT PicoKine ELISA Kit
Reactivity
Mouse
Synonyms
Serpin A1/AAT; Mouse Serpin A1/AAT PicoKine ELISA Kit; Mouse AAT; AAT elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse AAT
Assay Range
1.56ng/ml-100ng/ml
Sensitivity
<10pg/ml
Capture Antibody
A monoclonal antibody from rat.
Detection Antibody
A biotinylated polyclonal antibody from goat.
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |2876|13987|37231|2911|14122|4269
Standard Deviation: |158.18|713.3|1600.9|177.57|932.05|320.18
CV(%): |5.5%|5.1%|4.3%|6.1%|6.6%|7.5%
n|16|16|16|24|24|24
Mean(pg/ml): |2876|13987|37231|2911|14122|4269
Standard Deviation: |158.18|713.3|1600.9|177.57|932.05|320.18
CV(%): |5.5%|5.1%|4.3%|6.1%|6.6%|7.5%
Sample Data
Concentration (ng/ml): |0|1.56|3.13|6.25|12.5|25|50|100
OD: |0.042|0.207|0.368|0.659|1.054|1.369|1.645|2.069
OD: |0.042|0.207|0.368|0.659|1.054|1.369|1.645|2.069
Typical Standard Curve/Testing Data
(Mouse AAT PicoKine ELISA Kit standard curve)
Typical Standard Curve/Testing Data
(Mouse AAT PicoKine ELISA Kit standard curve)
Related Product Information for AAT elisa kit
Description: For quantitative detection of mouse AAT in cell culture supernates, serum and plasma (heparin, EDTA).
Background: Mouse AAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AAT has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E25-K413) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse AAT amount of sample captured in plate.
Background: Mouse AAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AAT has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E25-K413) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse AAT amount of sample captured in plate.
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Product Notes
The Mouse AAT (Catalog #AAA1751611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751611 ELISA Kit recognizes Mouse AAT. It is sometimes possible for the material contained within the vial of "Serpin A1/AAT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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