Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit | NOX1 elisa kit

Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit

Cat. Num. AAA2023718

• Reactivity: Human
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1); Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit; MOX1; GP91-2; NOH1; NADPH Oxidase 1; Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOX1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1).; No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
• Sequence Length: 563

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NOX1 elisa kit

Enzyme & Kinase; Neuroscience; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 16758500
• NCBI GeneID: 114243
• NCBI Accession #: NP_446135.1
• NCBI GenBank Nucleotide #: NM_053683.1
• Molecular Weight: 65,177 Da
• NCBI Official Full Name: NADPH oxidase 1
• NCBI Official Synonym Full Names: NADPH oxidase 1
• NCBI Official Symbol: Nox1
• NCBI Protein Information: NADPH oxidase 1
• UniProt Protein Name: NADPH oxidase 1
• Protein Family: NADPH oxidase
• UniProt Gene Name: Nox1
• UniProt Synonym Gene Names: Mox1; Noh1; NOX-1; MOX-1
• UniProt Entry Name: NOX1_RAT

NCBI Description

involved in superoxide generation and serum-stimulated growth [RGD, Feb 2006]

Uniprot Description

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; EC 1.-.-.-; Channel, misc.

Cellular Component: cytoplasm; early endosome; cell junction; endosome; NADPH oxidase complex

Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; voltage-gated proton channel activity

Biological Process: intracellular pH elevation; extracellular matrix organization and biogenesis; cell migration; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; regulation of systemic arterial blood pressure by renin-angiotensin; proton transport; response to reactive oxygen species; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; hydrogen peroxide metabolic process; angiogenesis; positive regulation of integrin biosynthetic process; superoxide release