Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) CLIA Kit | NOX1 clia kit

Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) CLIA Kit

Cat. Num. AAA2000911

• Reactivity: Rat
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1); Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) CLIA Kit; MOX1; GP91-2; NOH1; NADPH Oxidase 1; Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOX1 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1).; No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
• Sequence Length: 563

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 11.8pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX1 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) level in the sample or standard.;

Product Categories/Family for NOX1 clia kit

Enzyme & Kinase; Neuroscience; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 16758500
• NCBI GeneID: 114243
• NCBI Accession #: NP_446135.1
• NCBI GenBank Nucleotide #: NM_053683.1
• Molecular Weight: 65,177 Da
• NCBI Official Full Name: NADPH oxidase 1
• NCBI Official Synonym Full Names: NADPH oxidase 1
• NCBI Official Symbol: Nox1
• NCBI Protein Information: NADPH oxidase 1
• UniProt Protein Name: NADPH oxidase 1
• Protein Family: NADPH oxidase
• UniProt Gene Name: Nox1
• UniProt Synonym Gene Names: Mox1; Noh1; NOX-1; MOX-1
• UniProt Entry Name: NOX1_RAT

NCBI Description

involved in superoxide generation and serum-stimulated growth [RGD, Feb 2006]

Uniprot Description

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Channel, misc.; Oxidoreductase

Cellular Component: early endosome; cytoplasm; cell junction; endosome; NADPH oxidase complex

Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; voltage-gated proton channel activity

Biological Process: intracellular pH elevation; extracellular matrix organization and biogenesis; cell migration; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; regulation of systemic arterial blood pressure by renin-angiotensin; response to reactive oxygen species; proton transport; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; hydrogen peroxide metabolic process; angiogenesis; positive regulation of integrin biosynthetic process; superoxide release