Human NADPH oxidase 1 ELISA Kit | NOX1 elisa kit

Human NADPH oxidase 1 ELISA Kit

Cat. Num. AAA762073

• Reactivity: Human
• Synonyms: NADPH oxidase 1; Human NADPH oxidase 1 ELISA Kit; NOX1/NOH-1/Mitogenic oxidase 1 (MOX-1)/NADH/NADPH mitogenic oxidase subunit P65-MOX/Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1; NOX1 elisa kit

• Reactivity: Human
• Sequence Length: 563

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NOX1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NOX1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NOX1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NOX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NOX1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16758500
• NCBI GeneID: 114243
• NCBI Accession #: NP_446135.1
• NCBI GenBank Nucleotide #: NM_053683.1
• Molecular Weight: 65,177 Da
• NCBI Official Full Name: NADPH oxidase 1
• NCBI Official Synonym Full Names: NADPH oxidase 1
• NCBI Official Symbol: Nox1
• NCBI Protein Information: NADPH oxidase 1
• UniProt Protein Name: NADPH oxidase 1
• Protein Family: NADPH oxidase
• UniProt Gene Name: Nox1
• UniProt Synonym Gene Names: Mox1; Noh1; NOX-1; MOX-1
• UniProt Entry Name: NOX1_RAT

NCBI Description

involved in superoxide generation and serum-stimulated growth [RGD, Feb 2006]

Uniprot Description

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.-.-.-; Motility/polarity/chemotaxis; Channel, misc.

Cellular Component: cell junction; cytoplasm; early endosome; endosome; NADPH oxidase complex

Molecular Function: metal ion binding; protein binding; Rac GTPase binding; superoxide-generating NADPH oxidase activity; voltage-gated proton channel activity

Biological Process: angiogenesis; cell migration; extracellular matrix organization and biogenesis; hydrogen peroxide metabolic process; intracellular pH elevation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of smooth muscle cell proliferation; proton transport; regulation of systemic arterial blood pressure by renin-angiotensin; response to reactive oxygen species; signal transduction; superoxide metabolic process; superoxide release