Human Neuroserpin (NSP) ELISA Kit | NSP elisa kit

Human Neuroserpin (NSP) ELISA Kit

Cat. Num. AAA2020443

• Gene Names: SERPINI1; PI12; neuroserpin
• Reactivity: Human
• Synonyms: Neuroserpin (NSP); Human Neuroserpin (NSP) ELISA Kit; SERPINI1; PI12; Serpin Peptidase Inhibitor Clade I Member 1; Peptidase inhibitor 12; NSP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP).; No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.
• Sequence Length: 410

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.34ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NSP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NSP elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 4826904
• NCBI GeneID: 5274
• NCBI Accession #: NP_005016.1
• NCBI GenBank Nucleotide #: NM_005025.4
• UniProt Accession #: Q99574; Q6AHZ4; A8K217; D3DNP1
• Molecular Weight: 46,427 Da
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• NCBI Official Symbol: SERPINI1
• NCBI Official Synonym Symbols: PI12; neuroserpin
• NCBI Protein Information: neuroserpin
• UniProt Protein Name: Neuroserpin
• UniProt Gene Name: SERPINI1
• UniProt Synonym Gene Names: PI12; PI-12
• UniProt Entry Name: NEUS_HUMAN

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: regulation of cell adhesion; central nervous system development; peripheral nervous system development

Disease: Encephalopathy, Familial, With Neuroserpin Inclusion Bodies