Rabbit anti-Human Neuroserpin Polyclonal Antibody | anti-SerpinI1 antibody

Anti-Neuroserpin Antibody, Rabbit Polyclonal

Cat. Num. AAA8104896

• Gene Names: SERPINI1; PI12; neuroserpin
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Neuroserpin; Polyclonal Antibody; Anti-Neuroserpin Antibody; Rabbit Polyclonal; SerpinI1/Neuroserpin Antibody; Rabbit PAb; Antigen Affinity Purified; serpin peptidase inhibitor; clade I (neuroserpin); member 1; Anti-neuroserpin Antibody; Anti-PI12 Antibody; Anti-Serpin I1 Antibody; anti-SerpinI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SerpinI1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SerpinI1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human SerpinI1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human SerpinI1 (rh SerpinI1). SerpinI1 specific IgG was purified by Human SerpinI1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human SerpinI1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-SERPINI1 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain Tissue LysateLane B: Rat brain Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:52 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SerpinI1 antibody

Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein that belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).

References

Schrimpf SP. et al., 1997, Genomics. 40 (1): 55-62. Hill RM. et al., 2002, Ann N Y Acad Sci. 971: 406-15. Yepes M. et al., 2004, Thromb. Haemost. 91 (3): 457-64. Galliciotti G. et al., 2006, Front Biosci. 11: 33-45.

NCBI and Uniprot Product Information

• NCBI GI #: 4826904
• NCBI GeneID: 5274
• NCBI Accession #: NP_005016.1
• NCBI GenBank Nucleotide #: NM_005025.4
• UniProt Accession #: Q99574; Q6AHZ4; A8K217; D3DNP1
• Molecular Weight: 46,427 Da
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• NCBI Official Symbol: SERPINI1
• NCBI Official Synonym Symbols: PI12; neuroserpin
• NCBI Protein Information: neuroserpin; PI-12; serpin I1; peptidase inhibitor 12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1
• UniProt Protein Name: Neuroserpin
• Protein Family: Neuroserpin
• UniProt Gene Name: SERPINI1
• UniProt Synonym Gene Names: PI12; PI-12
• UniProt Entry Name: NEUS_HUMAN

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: regulation of cell adhesion; central nervous system development; peripheral nervous system development

Disease: Encephalopathy, Familial, With Neuroserpin Inclusion Bodies