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Typical Testing Data/Standard Curve (for reference only)

Human Neuroserpin (NSP) ELISA Kit | NSP elisa kit

Human Neuroserpin (NSP) ELISA Kit

Gene Names
SERPINI1; PI12; neuroserpin
Reactivity
Human
Synonyms
Neuroserpin (NSP); Human Neuroserpin (NSP) ELISA Kit; SERPINI1; PI12; Serpin Peptidase Inhibitor Clade I Member 1; Peptidase inhibitor 12; NSP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP).
No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.
Sequence Length
410
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
0.781-50ng/mL
Sensitivity
< 0.34ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NSP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,427 Da
NCBI Official Full Name
neuroserpin
NCBI Official Synonym Full Names
serpin family I member 1
NCBI Official Symbol
SERPINI1
NCBI Official Synonym Symbols
PI12; neuroserpin
NCBI Protein Information
neuroserpin
UniProt Protein Name
Neuroserpin
UniProt Gene Name
SERPINI1
UniProt Synonym Gene Names
PI12; PI-12

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed:9442076, PubMed:26329378, PubMed:19265707, PubMed:19285087, PubMed:11880376). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).

Research Articles on NSP

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Product Notes

The Human NSP serpini1 (Catalog #AAA2705078) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2705078 ELISA Kit recognizes Human NSP. It is sometimes possible for the material contained within the vial of "Neuroserpin (NSP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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