Western Blot (WB)
(Western blot analysis of extracts from various samples, using GCSc-gamma Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell; Lane 4: mouse lung; Lane 5: rat barin; )
Rabbit GCSc-gamma Polyclonal Antibody | anti-GCLC antibody
GCSc-gamma Antibody
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GCSc-gamma; Polyclonal Antibody; GCSc-gamma Antibody; EC 6.3.2.2; Gamma ECS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; Gclc; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN; anti-GCLC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GCSc-gamma antibody detects endogenous levels of total GCSc-gamma
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
599
Applicable Applications for anti-GCLC antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human GCSc-gamma
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using GCSc-gamma Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell; Lane 4: mouse lung; Lane 5: rat barin; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using GCSc-gamma Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell; Lane 4: mouse lung; Lane 5: rat barin; )
Western Blot (WB)
(Western blot analysis GCSc-gamma using RAW264.7 whole cell lysates)
Western Blot (WB)
(Western blot analysis GCSc-gamma using RAW264.7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of GCSc Antibody expression in RAW264.7 cells lysates.)
Western Blot (WB)
(Western blot analysis of GCSc Antibody expression in RAW264.7 cells lysates.)
Related Product Information for anti-GCLC antibody
Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Subunit Structure: Heterodimer of a catalytic heavy chain and a regulatory light chain.
Similarity: Belongs to the glutamate--cysteine ligase type 3 family.
Subunit Structure: Heterodimer of a catalytic heavy chain and a regulatory light chain.
Similarity: Belongs to the glutamate--cysteine ligase type 3 family.
References
Li L, Sun HY, Liu W, Zhao HY, Shao ML; Journal: Food Chem Toxicol. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. Zou Y, Wang J, Peng J, Wei H; Journal: Oxid Med Cell Longev. Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 72 kDa
Predicted: 73 kDa
Predicted: 73 kDa
NCBI Official Full Name
glutamate--cysteine ligase catalytic subunit isoform b
NCBI Official Synonym Full Names
glutamate-cysteine ligase catalytic subunit
NCBI Official Symbol
GCLC
NCBI Official Synonym Symbols
GCL; GCS; GLCL; GLCLC
NCBI Protein Information
glutamate--cysteine ligase catalytic subunit
UniProt Protein Name
Glutamate--cysteine ligase catalytic subunit
Protein Family
UniProt Gene Name
GCLC
UniProt Synonym Gene Names
GLCL; GLCLC
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Research Articles on GCLC
Similar Products
Product Notes
The GCLC gclc (Catalog #AAA9608867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCSc-gamma Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCSc-gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the GCLC gclc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCSc-gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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