Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin-Ie (MYO1E) Standard Curve.)
Rat Myosin-Ie (MYO1E) ELISA Kit | MYO1E elisa kit
Rat Myosin-Ie (MYO1E) ELISA Kit
Gene Names
Myo1e; MYR5; Myr3
Reactivity
Rat
Synonyms
Myosin-Ie (MYO1E); Rat Myosin-Ie (MYO1E) ELISA Kit; MYO1E; HuncM-IC; MGC104638; MYO1C; MYO1E variant protein; myosin-IC; MYO1E elisa kit
Reactivity
Rat
Specificity
Rat Myosin-Ie (MYO1E) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MYO1E. No significant cross-reactivity or interference between Rat MYO1E and analogues was observed.
Sequence Length
1107
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin-Ie (MYO1E) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myosin-Ie (MYO1E) Standard Curve.)
Related Product Information for MYO1E elisa kit
Description: This Rat Myosin-Ie (MYO1E) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1E in samples. An antibody specific for MYO1E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO1E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1E bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MYO1E encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in MYO1E are the cause of focal segmental glomerulosclerosis-6. MYO1E has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17.
Background: MYO1E encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in MYO1E are the cause of focal segmental glomerulosclerosis-6. MYO1E has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
unconventional myosin-Ie
NCBI Official Synonym Full Names
myosin IE
NCBI Official Symbol
Myo1e
NCBI Official Synonym Symbols
MYR5; Myr3
NCBI Protein Information
unconventional myosin-Ie
UniProt Protein Name
Unconventional myosin-Ie
Protein Family
UniProt Gene Name
Myo1e
UniProt Synonym Gene Names
Myr3
UniProt Entry Name
MYO1E_RAT
NCBI Description
member of the myosin I family; may function through cell-cell contact in F-actin-rich structures [RGD, Feb 2006]
Research Articles on MYO1E
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Product Notes
The Rat MYO1E myo1e (Catalog #AAA9712193) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712193 ELISA Kit recognizes Rat MYO1E. It is sometimes possible for the material contained within the vial of "Myosin-Ie (MYO1E), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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