Human Myeloperoxidase / MPO ELISA Kit | MPO elisa kit

Myeloperoxidase / MPO Human ELISA Kit

Cat. Num. AAA355337

• Reactivity: Human
• Applications: ELISA
• Synonyms: Myeloperoxidase / MPO; Myeloperoxidase / MPO Human ELISA Kit; Human Myeloperoxidase / MPO; MPO elisa kit

• Reactivity: Human

• Applicable Applications for MPO elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of MPO in human serum, body fluids, tissue lysates or cell culture supernatants.
• Samples: Human serum, body fluids, tissue lysates or cell culture supernatants
• Assay Type: Quantitative Sandwich
• Detection Range: 312 pg/ml-20,000 pg/ml
• Sensitivity: < 10 pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MPO elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MPO polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MPO polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MPO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MPO can be calculated.!!Background/Introduction: Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene which contains 12 exons and spans about 13 kb. Myeloperoxidase is most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in azurophilic granules of the neutrophil. The 150-kDa MPO protein is a dimer consisting of two 15-kDa light chains and two variable-weight glycosylated heavy chains bound to a prosthetic heme group. MPO produces hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion (Cl-) during the neutrophil's respiratory burst. It requires heme as a cofactor. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent.

NCBI and Uniprot Product Information

• NCBI GI #: 188658
• NCBI GeneID: 4353
• UniProt Accession #: P05164; Q14862; Q4PJH5; Q9UCL7; A1L4B8
• Molecular Weight: 83,869 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Catalytic activity: Cl- + H2O2 + H+ = HClO + H2O.Cl- + H2O2 = HOCl + 2 H2O.

Cofactor: Binds 1 calcium ion per monomer.Binds 1 heme B (iron-protoporphyrin IX) group covalently per monomer.

Subunit structure: Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Ref.13 Ref.21

Subcellular location: Lysosome.

Involvement in disease: Myeloperoxidase deficiency (MPOD) [MIM:254600]: A disorder characterized by decreased myeloperoxidase activity in neutrophils and monocytes that results in disseminated candidiasis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.24 Ref.25 Ref.26 Ref.27 Ref.28

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.