Typical Testing Data/Standard Curve (for reference only)
Rat Myeloperoxidase ELISA Kit | MPO elisa kit
Rat Myeloperoxidase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MPO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MPO antibody and an MPO-HRP conjugate. The assay sample and buffer are incubated together with MPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPO concentration since MPO from samples and MPO-HRP conjugate compete for the anti-MPO antibody binding site. Since the number of sites is limited, as more sites are occupied by MPO from the sample, fewer sites are left to bind MPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
Uniprot Description
MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.11.2.2
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule
Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding
Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; defense response; response to lipopolysaccharide; response to yeast; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging
Disease: Myeloperoxidase Deficiency; Alzheimer Disease
Research Articles on MPO
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Product Notes
The Rat MPO mpo (Catalog #AAA724567) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA724567 ELISA Kit recognizes Rat MPO. It is sometimes possible for the material contained within the vial of "Myeloperoxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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