Myeloperoxidase (MPO) Assay Kit | MPO assay kit

Myeloperoxidase (MPO) Colorimetric Activity Assay Kit

Cat. Num. AAA841548

• Synonyms: Myeloperoxidase (MPO); Myeloperoxidase (MPO) Colorimetric Activity Assay Kit; MPO assay kit

• Sequence Length: 745

• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MPO assay kit

Background/Introduction: Myeloperoxidase (MPO) is a peroxidase (EC 1.11.1.7) abundantly expressed in neutrophils. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion, Cl- (or halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as oxidizing agent. In MPO Assay Kit, HClO produced from H2O2 and Cl- reacts with taurine to generate taurine chloramine, which subsequently reacts with the TNB probe to eliminate color (lambda = 412 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of MPO. This kit can be used to detect MPO as low as 0.05 mU per well.

Product Categories/Family for MPO assay kit

Metabolism Assays; Oxidative stressMetabolism Assays; Metabolism Assays (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 188658
• NCBI GeneID: 4353
• Molecular Weight: 87,248 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; response to lipopolysaccharide; response to yeast; defense response; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease