Human Basic Fibroldast Growth Factor ELISA Kit | BFGF elisa kit

Human Basic Fibroldast Growth Factor ELISA Kit

Cat. Num. AAA720717

• Gene Names: FGF2; BFGF; FGFB; HBGF-2
• Reactivity: Human
• Synonyms: Basic Fibroldast Growth Factor; Human Basic Fibroldast Growth Factor ELISA Kit; Basic Fibroldast Growth Factor ELISA Kit (Human); BFGF elisa kit

• Reactivity: Human

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BFGF elisa kit

Intended Uses: This BFGF ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of BFGF in the sample, this BFGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus BFGF concentration. The concentration of BFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This BFGF enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for BFGF. Standards or samples are then added to the microtiter plate wells and BFGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BFGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BFGF are added to each well to "sandwich" the BFGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain BFGF and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for BFGF elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 8250666
• NCBI GeneID: 2247
• UniProt Accession #: P09038; O00527; P78443; Q16443; Q5PY50; Q7KZ11; Q7KZ72; Q9UC54; Q9UCS5; Q9UCS6; A4LBB8
• Molecular Weight: 30,770 Da
• NCBI Official Full Name: basic fibroblast growth factor
• NCBI Official Synonym Full Names: fibroblast growth factor 2 (basic)
• NCBI Official Symbol: FGF2
• NCBI Official Synonym Symbols: BFGF; FGFB; HBGF-2
• NCBI Protein Information: heparin-binding growth factor 2; prostatropin; OTTHUMP00000037513; basic fibroblast growth factor bFGF
• UniProt Protein Name: Heparin-binding growth factor 2
• UniProt Gene Name: FGF2
• UniProt Synonym Gene Names: FGFB
• UniProt Entry Name: FGF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq]

Uniprot Description

Function: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Ref.11

Subunit structure: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. Ref.20 Ref.21 Ref.22

Tissue specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. Ref.11 Ref.18

Post-translational modification: Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Miscellaneous: This protein binds heparin more strongly than does aFGF.

Sequence similarities: Belongs to the heparin-binding growth factors family.

Biophysicochemical propertiespH dependence:Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius. Ref.11Temperature dependence:Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius.

Sequence caution: The sequence AAA52448.1 differs from that shown. Reason: Frameshift at positions 25, 82, 98 and 133. The sequence AAB21432.2 differs from that shown. Reason: Frameshift at position 25. The sequence AAB21432.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence ABO43041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABO43041.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA28027.1 differs from that shown. Reason: Frameshift at positions 25 and 102. The sequence CAA28027.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA73868.1 differs from that shown. Reason: Frameshift at position 25. The sequence CAA73868.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence EAX05222.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAX05222.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.