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Typical Testing Data/Standard Curve (for reference only)

Human Mucin 17 (MUC17) ELISA Kit | MUC17 elisa kit

Human Mucin 17 (MUC17) ELISA Kit

Gene Names
MUC17; MUC3
Reactivity
Human
Synonyms
Mucin 17 (MUC17); Human Mucin 17 (MUC17) ELISA Kit; MUC3; Mucin Cell Surface Associated; Small intestinal mucin-3; MUC17 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mucin 17 (MUC17).
No significant cross-reactivity or interference between Mucin 17 (MUC17) and analogues was observed.
Sequence Length
4493
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
15.62-1000pg/mL
Sensitivity
< 6.0pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Mucin 17 (MUC17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Mucin 17 (MUC17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUC17 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 17 (MUC17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 17 (MUC17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 17 (MUC17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 17 (MUC17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MUC17 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
436,777 Da
NCBI Official Full Name
mucin-17
NCBI Official Synonym Full Names
mucin 17, cell surface associated
NCBI Official Symbol
MUC17
NCBI Official Synonym Symbols
MUC3
NCBI Protein Information
mucin-17
UniProt Protein Name
Mucin-17
Protein Family
UniProt Gene Name
MUC17
UniProt Synonym Gene Names
MUC3; MUC-17; MUC-3
UniProt Entry Name
MUC17_HUMAN

NCBI Description

Membrane mucins, such as MUC17, function in epithelial cells to provide cytoprotection, maintain luminal structure, provide signal transduction, and confer antiadhesive properties upon cancer cells that lose their apical/basal polarization.[supplied by OMIM, Apr 2004]

Uniprot Description

MUC17: Probably plays a role in maintaining homeostasis on mucosal surfaces. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: Golgi lumen; apical plasma membrane; integral to membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; extracellular matrix constituent, lubricant activity; PDZ domain binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cellular homeostasis; post-translational protein modification

Research Articles on MUC17

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Product Notes

The Human MUC17 muc17 (Catalog #AAA2020707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020707 ELISA Kit recognizes Human MUC17. It is sometimes possible for the material contained within the vial of "Mucin 17 (MUC17), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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