HAO1 recombinant protein

HAO1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121075

• Gene Names: HAO1; GOX; GOX1; HAOX1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: HAO1; HAO1 Protein; Human; Recombinant (His Tag); Human HAO1/GOX1 Protein (His Tag); GOX Protein; GOX1 Protein; HAOX1 Protein; hydroxyacid oxidase (glycolate oxidase) 1; HAO1 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tirs, 500mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu2-Ile370

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the native human HAO1 (NP_060015.1)(Leu 2-Ile 370) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HAO1 recombinant protein

Background: Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1, and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1/GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1/GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with the concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1/GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1/GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to an oxidative injury involving down-regulation of ROS-producing enzymes.

References

Jones J.M.et al., 2000, J. Biol. Chem. 275:12590-7. Recalcati,S. et al., 2001, J Cell Sci. 114 (Pt 9):1625-9. Recalcati,S. et al., 2003, Hepatology. 38 (5):1159-66. Murray M.S.et al., 2008, Biochemistry 47:2439-49. Bourhis J.M.et al., 2009, Acta Crystallogr. F 65:1246-53.

NCBI and Uniprot Product Information

• NCBI GI #: 11068137
• NCBI GeneID: 54363
• NCBI Accession #: NP_060015.1
• NCBI GenBank Nucleotide #: NM_017545.3
• UniProt Accession #: Q9UJM8
• NCBI Official Full Name: hydroxyacid oxidase 1
• NCBI Official Synonym Full Names: hydroxyacid oxidase 1
• NCBI Official Symbol: HAO1
• NCBI Official Synonym Symbols: GOX; GOX1; HAOX1
• NCBI Protein Information: hydroxyacid oxidase 1
• UniProt Protein Name: Hydroxyacid oxidase 1
• Protein Family: Hydroxyacid oxidase
• UniProt Gene Name: HAO1
• UniProt Synonym Gene Names: GOX1; HAOX1; HAOX1; GOX
• UniProt Entry Name: HAOX1_HUMAN

NCBI Description

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]

Uniprot Description

HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Protein type: EC 1.1.3.15; Oxidoreductase; Carbohydrate Metabolism - glyoxylate and dicarboxylate

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: peroxisomal matrix; peroxisome

Molecular Function: glycolate oxidase activity; FMN binding; glyoxylate oxidase activity; (S)-2-hydroxy-acid oxidase activity; receptor binding

Biological Process: glyoxylate metabolic process; response to oxidative stress; glycolate catabolic process; fatty acid alpha-oxidation