Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) Standard Curve.)
Human Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit | MAP3K6 elisa kit
Human Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit
Gene Names
MAP3K6; ASK2; MEKK6; MAPKKK6
Reactivity
Human
Synonyms
Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6); Human Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit; MAP3K6; RP11-4K3__A.7; ASK2; MAPKKK6; MEKK6; MGC125653; MGC20114; apoptosis signal regulating kinase 2; apoptosis signal-regulating kinase 2; MAP3K6 elisa kit
Reactivity
Human
Specificity
Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP3K6. No significant cross-reactivity or interference between Human MAP3K6 and analogues was observed.
Sequence Length
1288
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) Standard Curve.)
Related Product Information for MAP3K6 elisa kit
Description: This Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP3K6 in samples. An antibody specific for MAP3K6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP3K6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP3K6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP3K6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAP3K6 is a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. MAP3K6 and MAP3K5 share 45% amino acid sequence identity; their catalytic domains share 82% identity. The authors confirmed the interaction of MAP3K6 with MAP3K5 in vivo using coimmunoprecipitation. Recombinant MAP3K6 weakly activated the JNK but not the ERK or p38 kinase pathways.
Background: MAP3K6 is a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. MAP3K6 and MAP3K5 share 45% amino acid sequence identity; their catalytic domains share 82% identity. The authors confirmed the interaction of MAP3K6 with MAP3K5 in vivo using coimmunoprecipitation. Recombinant MAP3K6 weakly activated the JNK but not the ERK or p38 kinase pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 6 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 6
NCBI Official Symbol
MAP3K6
NCBI Official Synonym Symbols
ASK2; MEKK6; MAPKKK6
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 6
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 6
Protein Family
UniProt Gene Name
MAP3K6
UniProt Synonym Gene Names
ASK2; MAPKKK6; MEKK6
UniProt Entry Name
M3K6_HUMAN
NCBI Description
This gene encodes a serine/threonine protein kinase that forms a component of protein kinase-mediated signal transduction cascades. The encoded kinase participates in the regulation of vascular endothelial growth factor (VEGF) expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
MEKK6: a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Protein type: EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE11 family
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: cytoplasm
Molecular Function: MAP kinase kinase kinase activity; magnesium ion binding; ATP binding
Biological Process: activation of MAPKK activity; signal transduction; protein amino acid phosphorylation; activation of JNK activity
Research Articles on MAP3K6
Similar Products
Product Notes
The Human MAP3K6 map3k6 (Catalog #AAA9715898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715898 ELISA Kit recognizes Human MAP3K6. It is sometimes possible for the material contained within the vial of "Mitogen-Activated Protein Kinase Kinase Kinase 6 (MAP3K6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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