Rat Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit | MAP3K6 elisa kit

Rat Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit

Cat. Num. AAA2705160

• Gene Names: MAP3K6; ASK2; MEKK6; MAPKKK6
• Reactivity: Rat
• Synonyms: Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6); Rat Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) ELISA Kit; MAPKKK6; ASK2; MEKK6; Apoptosis Signal Regulating Kinase 2; MAP3K6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6).; No significant cross-reactivity or interference between Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) and analogues was observed.
• Sequence Length: 1,280

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAP3K6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45827809
• NCBI GeneID: 9064
• NCBI Accession #: NP_004663.3
• NCBI GenBank Nucleotide #: NM_004672.4
• UniProt Accession #: O95382; Q59HF4; Q5SSD4; Q75PK3; Q96B75; A2ACE8; A2VDG4; A2VDG5
• Molecular Weight: 141,648 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 6 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 6
• NCBI Official Symbol: MAP3K6
• NCBI Official Synonym Symbols: ASK2; MEKK6; MAPKKK6
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 6
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 6
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K6
• UniProt Synonym Gene Names: ASK2; MAPKKK6; MEKK6

NCBI Description

This gene encodes a serine/threonine protein kinase that forms a component of protein kinase-mediated signal transduction cascades. The encoded kinase participates in the regulation of vascular endothelial growth factor (VEGF) expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.