Goat anti-Human, Rat MAP3K6 Polyclonal Antibody | anti-MAP3K6 antibody

MAP3K6 (Mitogen-activated Protein Kinase Kinase Kinase 6, MAPKKK6, Apoptosis Signal-regulating Kinase 2, ASK2, MEKK6)

Cat. Num. AAA629132

• Gene Names: MAP3K6; ASK2; MEKK6; MAPKKK6
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: MAP3K6; Polyclonal Antibody; MAP3K6 (Mitogen-activated Protein Kinase Kinase Kinase 6; MAPKKK6; Apoptosis Signal-regulating Kinase 2; ASK2; MEKK6); Anti -MAP3K6 (Mitogen-activated Protein Kinase Kinase Kinase 6; anti-MAP3K6 antibody

• Host: Goat
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAP3K6. Species crossreactivity
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-MAP3K6 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:8000; Western Blot: 0.3-1ug/ml detects ~140kD band observed in Rat Heart lysates (calculated MW of 143kD according to Human NP_004663.3 and Rat NP_001101379.1).
• Immunogen: Synthetic peptide corresponding to C-RAILAQRAGSTP, from the C-Terminus of human MAP3K6 (NP_004663.3).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS629132(0.3µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-MAP3K6 antibody

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Product Categories/Family for anti-MAP3K6 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 45827809
• NCBI GeneID: 9064
• NCBI Accession #: NP_004663.3
• NCBI GenBank Nucleotide #: NM_004672.3
• UniProt Accession #: O95382; Q59HF4; Q5SSD4; Q75PK3; Q96B75; A2ACE8; A2VDG4; A2VDG5; JE0363
• Molecular Weight: 142,596 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 6
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 6
• NCBI Official Symbol: MAP3K6
• NCBI Official Synonym Symbols: ASK2; MEKK6; MAPKKK6
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 6; apoptosis signal regulating kinase 2; apoptosis signal-regulating kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 6
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K6
• UniProt Synonym Gene Names: ASK2; MAPKKK6; MEKK6
• UniProt Entry Name: M3K6_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK6: a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Protein type: Protein kinase, STE; EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm

Molecular Function: MAP kinase kinase kinase activity; magnesium ion binding; ATP binding

Biological Process: activation of MAPKK activity; signal transduction; protein amino acid phosphorylation; activation of JNK activity