Western Blot (WB)
(Western Blot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS605447 (1:5000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates EZH2 (~98kD).)
Mouse anti-Human, Mouse EZH2 Monoclonal Antibody | anti-EZH2 antibody
EZH2 (Histone-lysine N-methyltransferase EZH2, Enhancer of Zeste Homolog 2, ENX-1, Lysine N-methyltransferase 6)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 1:5000
Western Blot (WB)
(Western Blot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS605447 (1:5000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates EZH2 (~98kD).)
Western Blot (WB)
(Western Blot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS605447 (1:5000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates EZH2 (~98kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Uniprot Description
EZH2: a histone H3 Lys 27 (H3K27) methyltransferase and Polycomb group protein with oncogenic activity. A master regulatory protein that plays a critical role in development. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates K9 and K27 of histone H3, leading to transcriptional repression of the affected target genes. PRC2 includes the Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells (ESCs). The recruitment of PRC2 to promoters in ES cells is interdependent on Jarid 2. ESCs lacking the PRC2 component EED are deficient in Jarid2 promoter activity, while the knockdown of Jarid 2 reduces PRC2 at its target promoters. Able to mono-, di- and trimethylate K27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, p14ARF and retinoic acid target genes. Binds ATRX via the SET domain. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Expression decreases during senescence of embryonic fibroblasts. Expression peaks at the G1/S phase boundary. Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53. It has been reported that phosphorylation by AKT1 on S21 reduces methyltransferase activity.
Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43
Chromosomal Location of Human Ortholog: 7q35-q36
Cellular Component: nucleoplasm; ESC/E(Z) complex; pronucleus; nuclear chromatin; cytoplasm; nucleus
Molecular Function: histone methyltransferase activity; protein binding; DNA binding; RNA binding; sequence-specific DNA binding; chromatin DNA binding; protein-lysine N-methyltransferase activity; chromatin binding; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: negative regulation of striated muscle cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of gliogenesis; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; satellite cell compartment self-renewal involved in skeletal muscle regeneration; regulation of gene expression, epigenetic; regulation of cell proliferation; positive regulation of MAP kinase activity; cerebellar cortex development; negative regulation of retinoic acid receptor signaling pathway; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of epidermal cell differentiation; DNA methylation; gene expression; negative regulation of transcription, DNA-dependent
Disease: Weaver Syndrome
Research Articles on EZH2
2. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
3. The over-expressions of the EZH2 protein and EZH2 mRNA may play an important role in the pathogenesis and progression of prostate cancer.
4. findings suggest that high expression of EZH2 correlates with tumor aggressiveness and adverse patient outcome in ESCC treated with definitive chemoradiotherapy
5. found that ERG disrupts androgen receptor (AR) signaling by inhibiting AR expression, binding to and inhibiting AR activity at gene-specific loci, and inducing repressive epigenetic programs via direct activation of the H3K27 methyltransferase EZH2
6. EZH2 and BMI-1 are upregulated in osteosarcoma. EZH2 and BMI-1 may be useful targets for cancer immunotherapy of osteosarcoma, although knock-down of EZH2 and BMI-1 could not prevent osteosarcoma growth.
7. There is a strong correlation between EZH2 overexpression and advanced stage, aggressive prostate cancers.
8. Omega-3 PUFAs degrade EZH2 via posttranslational mechanisms. EZH2 downregulation by omega-3 PUFAs decreases histone 3 lysine 27 trimethylation activity of EZH2 & upregulation of E-cadherin & IGFBP3, which are known targets of EZH2.
9. Although EZH2 does not influence the basal level of apoptotic activity, its overexpression protects the tumor cells against the proapoptotic action of (-)-epigallocatechin-3-gallate.
10. Data show that EZH2 is a crucial regulator of GAGE2 expression.
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Product Notes
The EZH2 ezh2 (Catalog #AAA605447) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EZH2 (Histone-lysine N-methyltransferase EZH2, Enhancer of Zeste Homolog 2, ENX-1, Lysine N-methyltransferase 6) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EZH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:5000. Researchers should empirically determine the suitability of the EZH2 ezh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZH2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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