Human Melatonin Receptor 1A (MTNR1A) ELISA Kit | MTNR1A elisa kit

Human Melatonin Receptor 1A (MTNR1A) ELISA Kit

Cat. Num. AAA454459

• Gene Names: MTNR1A; MT1; MEL-1A-R
• Reactivity: Human
• Synonyms: Melatonin Receptor 1A (MTNR1A); Human Melatonin Receptor 1A (MTNR1A) ELISA Kit; MT1 MEL-1A-R; MTNR1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTNR1A. No significant cross-reactivity or interference between MTNR1A and analogues was observed.
• Sequence Length: 350

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.219ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTNR1A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTNR1A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTNR1A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MTNR1A in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MTNR1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MTNR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTNR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MTNR1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5174593
• NCBI GeneID: 4543
• NCBI Accession #: NP_005949.1
• NCBI GenBank Nucleotide #: NM_005958.4
• UniProt Accession #: P48039; A0AVC5; B0M0L2
• Molecular Weight: 39,375 Da
• NCBI Official Full Name: melatonin receptor type 1A
• NCBI Official Synonym Full Names: melatonin receptor 1A
• NCBI Official Symbol: MTNR1A
• NCBI Official Synonym Symbols: MT1; MEL-1A-R
• NCBI Protein Information: melatonin receptor type 1A
• UniProt Protein Name: Melatonin receptor type 1A
• Protein Family: Melatonin receptor
• UniProt Gene Name: MTNR1A
• UniProt Synonym Gene Names: Mel-1A-R; Mel1a receptor

NCBI Description

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]

Uniprot Description

High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.