S100A4 recombinant protein

Recombinant Human S100A4

Cat. Num. AAA4159768

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Purity: >95% as determined by SDS-PAGE
• Synonyms: S100A4; Recombinant Human S100A4; Protein S100-A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4; CAPL; MTS1; Recombinant Human S100 Calcium Binding Protein A4 (S100A4); S100A4 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered PBS solution and 1.0 mM DTT.
• Sequence Length: 101

• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for S100A4 recombinant protein

S100A4 is a calcium-binding protein related to the S100 family of proteins. It is known also as a growth-related protein, CAPL. It is expressed at high levels in different mouse metastatic cells and in normal tissues (thymus, spleen) and activated macrophages, T cells, neutrophils, monocytes, macrophages.

NCBI and Uniprot Product Information

• NCBI GI #: 115601
• NCBI GeneID: 6285
• UniProt Accession #: P26447; P04271
• Molecular Weight: 12.0 kDa
• NCBI Official Full Name: Protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-A4
• Protein Family: Protein
• UniProt Gene Name: S100A4
• UniProt Synonym Gene Names: CAPL; MTS1
• UniProt Entry Name: S10A4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; calcium ion binding; actin binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial to mesenchymal transition