Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 210442 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit)
Rabbit anti-Human DDX58 Polyclonal Antibody | anti-DDX58 antibody
DDX58 (DDX58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I, Retinoic Acid Inducible Gene 1 Protein) (HRP)
Gene Names
DDX58; RIGI; RIG-I; RLR-1
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
DDX58; Polyclonal Antibody; DDX58 (DDX58; DKFZp434J1111; DKFZp686N19181; FLJ13599; RIG-I; Retinoic Acid Inducible Gene 1 Protein) (HRP); anti-DDX58 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DDX58.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-DDX58 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:20-1:100
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to human DDX58.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 210442 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 210442 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-DDX58 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
925
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX58
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
NCBI Official Symbol
DDX58
NCBI Official Synonym Symbols
RIGI; RIG-I; RLR-1
NCBI Protein Information
probable ATP-dependent RNA helicase DDX58; RIG-1; RNA helicase RIG-I; DEAD box protein 58; RIG-I-like receptor 1; retinoic acid inducible gene I; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; DEAD/H (Asp-Glu-Ala-Asp/His)
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX58
Protein Family
UniProt Gene Name
DDX58
UniProt Synonym Gene Names
RLR-1; RIG-1; RIG-I
UniProt Entry Name
DDX58_HUMAN
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Product Notes
The DDX58 ddx58 (Catalog #AAA6413894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX58 (DDX58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I, Retinoic Acid Inducible Gene 1 Protein) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX58 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:20-1:100 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DDX58 ddx58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX58, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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