Mouse Melatonin receptor type 1A (MTNR1A) ELISA Kit | MTNR1A elisa kit

Mouse Melatonin receptor type 1A (MTNR1A) ELISA Kit

Cat. Num. AAA7236751

• Gene Names: MTNR1A; MT1; MEL-1A-R
• Reactivity: Mouse
• Synonyms: Melatonin receptor type 1A (MTNR1A); Mouse Melatonin receptor type 1A (MTNR1A) ELISA Kit; MTNR1A elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This MTNR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MTNR1A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTNR1A elisa kit

Principle of the assay: MTNR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MTNR1A antibody and an MTNR1A-HRP conjugate. The assay sample and buffer are incubated together with MTNR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTNR1A concentration since MTNR1A from samples and MTNR1A-HRP conjugate compete for the anti-MTNR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by MTNR1A from the sample, fewer sites are left to bind MTNR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTNR1A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MTNR1A elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 5174593
• NCBI GeneID: 4543
• NCBI Accession #: NP_005949.1
• NCBI GenBank Nucleotide #: NM_005958.3
• Molecular Weight: 39,375 Da
• NCBI Official Full Name: melatonin receptor type 1A
• NCBI Official Synonym Full Names: melatonin receptor 1A
• NCBI Official Symbol: MTNR1A
• NCBI Official Synonym Symbols: MT1; MEL-1A-R
• NCBI Protein Information: melatonin receptor type 1A; mel1a receptor
• UniProt Protein Name: Melatonin receptor type 1A
• Protein Family: Melatonin receptor
• UniProt Gene Name: MTNR1A
• UniProt Synonym Gene Names: Mel-1A-R; Mel1a receptor
• UniProt Entry Name: MTR1A_HUMAN

NCBI Description

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]

Uniprot Description

MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; hormone binding; melatonin receptor activity

Biological Process: circadian rhythm; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cGMP biosynthetic process; mating behavior