Chicken Melatonin (MT) ELISA Kit | MT elisa kit

Chicken Melatonin (MT) ELISA Kit

Cat. Num. AAA262913

• Gene Names: MTNR1A; MT1; MEL-1A-R
• Reactivity: Chicken
• Synonyms: Melatonin (MT); Chicken Melatonin (MT) ELISA Kit; MT elisa kit

• Reactivity: Chicken
• Sequence Length: 350

• Samples: Chicken serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/mL-15.6 pg/mL
• Sensitivity: Up to 5 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Chicken MT monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 5174593
• NCBI GeneID: 4543
• NCBI Accession #: NP_005949.1
• NCBI GenBank Nucleotide #: NM_005958.3
• Molecular Weight: 39,375 Da
• NCBI Official Full Name: melatonin receptor type 1A
• NCBI Official Synonym Full Names: melatonin receptor 1A
• NCBI Official Symbol: MTNR1A
• NCBI Official Synonym Symbols: MT1; MEL-1A-R
• NCBI Protein Information: melatonin receptor type 1A; mel1a receptor
• UniProt Protein Name: Melatonin receptor type 1A
• UniProt Gene Name: MTNR1A
• UniProt Synonym Gene Names: Mel-1A-R; Mel1a receptor
• UniProt Entry Name: MTR1A_HUMAN

NCBI Description

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]

Uniprot Description

MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; hormone binding; melatonin receptor activity

Biological Process: circadian rhythm; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cGMP biosynthetic process; mating behavior