Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using PATE3 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit PATE3 Polyclonal Antibody | anti-PATE3 antibody
PATE3 Polyclonal Antibody
Gene Names
PATE3; HEL-127; PATE-DJ
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
PATE3; Polyclonal Antibody; PATE3 Polyclonal Antibody; HEL-127; PATE-DJ; anti-PATE3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
98
Applicable Applications for anti-PATE3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PATE3
Calculated Molecular Weight
12KDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using PATE3 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using PATE3 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-PATE3 antibody
PATE3 (prostate and testis expressed protein 3), also known as PATE-DJ or HEL-127, is a 98 amino acid protein that contains one UPAR/Ly6 domain and belongs to the PATE family. PATE3 is a secreted protein that is expressed in prostate and testis. The gene that encodes PATE3 consists of around 3,490 bases and maps to human chromosome 11p15.5. Chromosome 11, which comprises approximately 4% of the human genome, is considered a gene and disease association-dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and thalassemia are caused by HBB gene mutations, while Wilms' tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are also associated with defects in chromosome 11-encoded genes.
Product Categories/Family for anti-PATE3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
98
NCBI Official Full Name
Prostate and testis expressed protein 3
NCBI Official Synonym Full Names
prostate and testis expressed 3
NCBI Official Symbol
PATE3
NCBI Official Synonym Symbols
HEL-127; PATE-DJ
NCBI Protein Information
prostate and testis expressed protein 3; PATE-like protein DJ; acrosomal vesicle protein HEL-127; secreted TFP/Ly-6/uPAR protein PATE-DJ
UniProt Protein Name
Prostate and testis expressed protein 3
Protein Family
UniProt Gene Name
PATE3
UniProt Synonym Gene Names
PATE-DJ
UniProt Entry Name
PATE3_HUMAN
Uniprot Description
PATE3: Belongs to the PATE family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: extracellular region
Research Articles on PATE3
Similar Products
Product Notes
The PATE3 pate3 (Catalog #AAA127711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PATE3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PATE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the PATE3 pate3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PATE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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