Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit | LPCAT3 elisa kit

Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit

Cat. Num. AAA9715455

• Gene Names: LPCAT3; C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
• Reactivity: Human
• Synonyms: Lysophospholipid Acyltransferase 5 (LPCAT3); Human Lysophospholipid Acyltransferase 5 (LPCAT3) ELISA Kit; LPCAT3; C3F; MBOAT5; OACT5; nessy; O-acyltransferase (membrane bound) domain containing 5; membrane bound O-acyltransferase domain containing 5; putative protein similar to nessy; LPCAT3 elisa kit

• Reactivity: Human
• Specificity: Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT3. No significant cross-reactivity or interference between Human LPCAT3 and analogues was observed.
• Sequence Length: 487

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Lysophospholipid acyltransferase 5 (LPCAT3) Standard Curve.)

Related Product Information for LPCAT3 elisa kit

Description: This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3 in samples. An antibody specific for LPCAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPCAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx). RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells. Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.

NCBI and Uniprot Product Information

• NCBI GI #: 42542394
• NCBI GeneID: 10162
• NCBI Accession #: NP_005759.4
• NCBI GenBank Nucleotide #: NP_005759.4
• UniProt Accession #: Q6P1A2
• NCBI Official Full Name: lysophospholipid acyltransferase 5
• NCBI Official Synonym Full Names: lysophosphatidylcholine acyltransferase 3
• NCBI Official Symbol: LPCAT3
• NCBI Official Synonym Symbols: C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
• NCBI Protein Information: lysophospholipid acyltransferase 5
• UniProt Protein Name: Lysophospholipid acyltransferase 5
• Protein Family: Lysophospholipid acyltransferase
• UniProt Gene Name: LPCAT3
• UniProt Synonym Gene Names: MBOAT5; OACT5; LPLAT 5; LPCAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; LPSAT; Lyso-PS acyltransferase; O-acyltransferase domain-containing protein 5
• UniProt Entry Name: MBOA5_HUMAN

Uniprot Description

MBOAT5: Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3- phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero- 3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn- glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Belongs to the membrane-bound acyltransferase family.

Protein type: Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.n6; EC 2.3.1.23

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity

Biological Process: glycerophospholipid biosynthetic process; phospholipid metabolic process